MEDTOX Abstract:  Clinical Evaluation of Chromium, Cobalt and Titanium by ICP-MS For Metal on Metal Implants 
Purpose:  To develop and validate a rapid and accurate method for the concurrent quantitation of chromium, cobalt, and titaniumin human whole blood and serum samples for the purpose of monitoring metal ion levels in patients with metal prosthetic implants.

MEDTOX Research Article:  Analysis of total and transferrin-bound iron in human serum for pharmacokinetic studies of iron–sucrose formulations
Background:  Patients with iron-deficiency anemia benefit from intravenous iron therapies. Development of these pharmaceutical agents requires pharmacokinetic studies monitoring levels of both the administered agent and transferrin-bound iron (TBI). Successful pharmacokinetic methods must discriminate iron species. Results: Routine colorimetric procedures were used to reliably measure total iron and TBI following iron–sucrose administration. Iron was liberated from iron–sucrose allowing the determination of all circulating iron. Solid-phase sample processing allowed the measurement of TBI. Circulating iron–sucrose could then be calculated as the difference between total iron and TBI. Conclusion: A reproducible and robust spectrophotometric method was developed and validated for measuring total iron and TBI in human serum following iron–sucrose therapy.

MEDTOX Abstract:  Digital image analysis improves inflammatory cell subset quantitation
Purpose:  Quantitation of inflammatory cell subsets in skin biopsies is often useful in evaluation of treatment response in clinical trials involving dermatopathic conditions. However, manual counting of immunohistochemically stained biopsies may be time consuming and subject to interobserver variability. We report on the use of digital analysis software which allows rapid quantitation of positively staining cells on IHC stained slides from skin punch biopsies.  

MEDTOX Poster: Concurrent Spectrophotometric Determinations of Total Iron and Transferrin-bound Iron in Human Serum
Purpose:  To develop and validate a chemical spectrophotometric method for the concurrent determination of total circulating iron (iron sucrose, transferrinbound iron and free iron) and the selective measurement of transferrin-bound iron (removing contributions from free and sucrose–bound iron) in human serum from subjects that received an  injectable iron sucrose product.

MEDTOX Poster:  High Throughput Screening and Quantitation of Prescription/Pain Medications in Human Urine by UPLC/MS/MS
Objective:  To screen for the presence of 102 compounds and simultaneously quantify 48 common prescription compounds (27 opiates and opioids, 14 benzodiazepines and 7 drugs of abuse) in human urine using a technique based upon ultra-high performance liquid chromatography tandem mass spectrometry (UPLC/MS/MS).

MEDTOX Poster: Quantification of Soluble Proteins Associated with Human Inflammation in Human Plasma by Flow Cytometric Analysis
Purpose: Validation of a customized cytokine panel designed to monitor the inflammatory profile of patients with acute renal failure receiving a novel therapy. The panel included interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-12p70 (IL-12p70), interferon gamma (IFN-gamma) and tumor necrosis factor (TNF).

MEDTOX Poster:   Therapeutic Drug Monitoring of Anti-Epileptic Drugs Using Dried Blood Spot
Purpose:  To quantify 10 different antiepileptic drugs by high performance liquid chromatography with electrospray ionization tandem mass spectrometry (LC/MS/MS) from dried capillary blood spots (DBS) collected on filter paper and then correlate the DBS determination to traditional plasma results.

MEDTOX Poster:  Bioanalysis of Acetyl Salicylic Acid, Salicylic Acid and Thromboxane B2, as a Biomarker of Aspirin Activity Using LC/MS/MS
Purpose:   Develop bioanalytical LC/MS/MS methods to analyze Acetyl salicylic acid (ASA, Aspirin) and salicylic acid (SA) and develop an LC/MS/MS method to monitor the anti-thrombin activity of aspirin by using Thromboxane B2 as a biomarker.

MEDTOX Poster:  Comparison of Anticonvulsant Concentrations Measured in Plasma and Whole Blood Spots Collected on Filter Paper
We have previously reported the development of an analytical method which provides simultaneous quantitation of 13 different antiepileptic compounds by high performance liquid chromatography with electrospray ionization tandem mass spectrometry (LC/MS/MS). This method requires minimal sample volumes and has been validated for dried capillary whole blood spots collected on filter paper. Utilization of this sample type eliminates the need for phlebotomy, allowing samples to be obtained by relatively untrained caregivers or patients. This provides a means for caregivers to collect TDM samples at crucial time points in non-medical settings, as well as facilitating routine sample collection.

MEDTOX Poster: Simultaneous Extraction and Sequential Quantification of Oxycodone and Ibuprofen in Human Plasma Using Mixed Mode Solid Phase Extraction and LC/MS/MS
Purpose: To extract and sequentially quantitate Oxycodone and Ibuprofen present at exponentially different levels in Human Plasma using Mixed Mode Solid Phase Extraction and LC/MS/MS.